Hydrotropic Solvents Used For Extraction Techniques Of Barleria Elegans L. Leaves

Rakesh Pal Singh; D. B. Joshi; R. S. Bhadauria

doi:10.18535/ijsrm/v10i04.mp01

Abstract

Plant species belonging to the family Acanthaceae are globally known to possess various medicinal properties and have cultural and economic importance in both traditional medicine and horticulture. They are important to both animals and humans and are used as food or for ornamental purposes worldwide. Barleria is the third largest genus in the family Acanthaceae. A few of the highly important and reported species of Barleria include B. prionitis, B. cristata, B. grandiflora, and B. lupulina. The flowers, leaves, stems, roots, and seed extracts of plants belonging to this genus are rich in bioactive compounds and have exhibited significant medicinal potential for the treatment of various ailments and infections.

Hydrotropic agents are stated as ionic organic salts which help to increase or decrease the solubility of solute in a given solvent via ‘salt in’ or ‘salt out’ effects, respectively. Salts which show ‘salt in’ of non-electrolytes are called “hydrotropic salts” and the phenomenon is known as “hydrotropism. There are about 45,000 plant species in India, with concentrated hotspots in the region of Eastern Himalayas.Entire plants of Barleriaelegans collected from Shrinathji Institute of pharmacy, Nathdwara and the plant was authenticated by Dr. R.L. Bhardwaj. The leaves of Barleriaelegans plant was characterized by its morphological features like colour, shape, size and surface characteristics has been studies. The plant materials (500gm) were initially defatted with petroleum ether and then extracted with blends RP-1 to RP-6 using a Soxhlet apparatus. The yield of the plant extracts measured about 20 g each after evaporating the solvent using water bath. As per result of TLC, we found that the hydrotropic blends solution aurecoferindly and cost effective method for the extraction purpose. All the phytoconstitutents which was earlier reported in previous literature by the use of organic solvents which were also present for the use of hydrotropic solvents.

Keywords

BarleriaelegansHydrotropyAlkaloidsTanninsSaponinHPTLC

References

Banerjee D, Maji A, BanerjiP. BarleriaprionitisLinn: A review of its traditional uses, phytochemistry, pharmacology and toxicity. Res J Phytochem2012;6:31-41.Google Scholar ↗
VasoyaU. Pharmacognostical and physicochemical studies on the leaves of Barleriaprionitis(L). Int J Pharm Sci Res2012;3(7):2291.Google Scholar ↗
Sharma P, Shrivastava B, Sharma GN, Jadhav HR. Phytochemical and ethenomedicinal values of BarleriaprionitisL: An overview. J Harmonized Res Pharm2013;2(3):190-9.Google Scholar ↗
Jain S, Jain R, Singh R. Ethnobotanical survey of Sariska and Siliserh regions from Alwar district of Rajasthan, India. EthnobotLeafl 2009;1:21.Google Scholar ↗
ShendageSM,YadavSR.RevisionofthegenusBarleria(Acanthaceae) in India. Rheedea 2010;20(2):81-130.Google Scholar ↗
ShuklaP, Singh A, Gawri S, Alexande A, Sonwane S. In vitro propagation of BarleriaprionitisLinn and its antibacterial activity. IntJ Pharm Prof Res2011;2:198-200.Google Scholar ↗
KhareCP. Indian Medicinal Plants: An Illustrated Dictionary. 1sted. New York: Springer Sciences; 2007. p.82-3.Google Scholar ↗
ChopraRN,NayarSL,ChopraIC.GlossaryofIndianMedicinalPlants. New Delhi: CSIR; 1956. p.33-4.Google Scholar ↗
AmbastaSP. The Useful Plants of India. New Delhi: CSIR;1986.Google Scholar ↗
BhallaNP, Sahu TR, Mishra GP, Dakwala RN. . J EconTaxon BotGoogle Scholar ↗
;3:23.Google Scholar ↗
Jain SK,Defillips RA. Medicinal Plants of India. New Delhi: CSIR; 1991.Google Scholar ↗
Patel BK, Chandel BS, Chauhan HC, Patel KB, Parth FM, Patel MV, et al. Evaluation of antibacterial activities of BarleriaprionitisLinn. Afr J Microbiol Res 2015;9Suppl30:1840-8.Google Scholar ↗
Kumar U, Ahmed F, KhanojiaP, Kukreja K, Kumari S, Bhat RA. Exploration of antioxidant and antibacterial activity of BarleriaprionitisLinn. Int J CurrMicrobiolApplSci2013;2(12):585-91.Google Scholar ↗
AiswaryaT, Ravikumar R. A comparitive study on phytochemical analysis, antibacterial activity and antioxidant activity of Barleriaprionitisleavesextractofpetroleumetherandethanolextract.IntJGoogle Scholar ↗
Kalhari KS, Zahida S, Udenigwea CC, Akhtara S, Ata A, Samarasekera R. Glutathione S-transferase, acetylcholinesterase inhibitory and antibacterial activities of chemical constituents of Barleriaprionitis. Z Naturforsch2007;62(b):580-6.Google Scholar ↗
Aneja KR, Joshi R, Sharma C. Potency of BarleriaprionitisL, bark extracts against oral diseases causing strains of bacteria and fungi of clinical origin. N Y Sci J2010;3:5-12.Google Scholar ↗
Khare D, Tiwari KL. Effect of leaf exudates and leaf tissue sap of BarleriaprionitisL,onsporegerminationofsomespicesofanamorphic fungi. Int J Res EngApplSci2016;6(4):1-7.Google Scholar ↗
ChenJL,BlancP,StoddartCA,BoganM,RozhonEJ,ParkinsonN,etal. New iridoids from the medicinal plant Barleriaprionitiswith potent activity against respiratory syncytial virus. J Nat Prod1998;61:1295-7.Google Scholar ↗
KaurR,KaurG,KapoorA.Preliminaryphytochemicalscreeningandin vitro anthelmintic activity of whole plant extracts of BarleriaprionitisLinn,againstearthworms:Pheretimaposthuma.WorldJPharmPharmSci 2015;4Suppl7:1340-7.Google Scholar ↗
Chavana CB, Hogadeb MG, Bhingea SD, Kumbhara M, Tamboli A. In vitro anthelmintic activity of fruit extract of BarleriaprionitisLinn, against Pheretimaposthuma. Int J Pharm PharmSci2010;2(3):49-50.Google Scholar ↗
Gupta RS, Kumar P, Dixit VP, DobhalMP. Antifertility studies of the rootextractoftheBarleriaprionitisLinninmalealbinoratswithspecial reference to testicular cell population dynamics. J Ethnopharmacol 2000;70:111-7.Google Scholar ↗
PradhanDK,MishraMR,MishraA,PandaAK,BeheraR,JhaS,etal. A comprehensive review of plants used as contraceptives. Int J Pharm Sci Res2012;4:148-55.Google Scholar ↗
RavichandranV, Arunachalam G, Subramanian N, Suresh B. Contraception and its significance in traditional system of medicines. Int J Pharm Sci 2009;1Suppl1:1-21.Google Scholar ↗
Kaur R, Sharma A, Kumar R, Kharb R. Rising trends towards herbal contraceptives. J Nat Prod Plant Resour2011;1(4):5-12.Google Scholar ↗
Singh K, Gupta RS. Antifertility activity of β-sisolated from Barleriaprionitis(l), roots in male albino rats. Int J Pharm PharmSci 2016;8(5):88-96.Google Scholar ↗

[refR-1] Banerjee D, Maji A, BanerjiP. BarleriaprionitisLinn: A review of its traditional uses, phytochemistry, pharmacology and toxicity. Res J Phytochem2012;6:31-41.Google Scholar ↗

[refR-2] VasoyaU. Pharmacognostical and physicochemical studies on the leaves of Barleriaprionitis(L). Int J Pharm Sci Res2012;3(7):2291.Google Scholar ↗

[refR-3] Sharma P, Shrivastava B, Sharma GN, Jadhav HR. Phytochemical and ethenomedicinal values of BarleriaprionitisL: An overview. J Harmonized Res Pharm2013;2(3):190-9.Google Scholar ↗

[refR-4] Jain S, Jain R, Singh R. Ethnobotanical survey of Sariska and Siliserh regions from Alwar district of Rajasthan, India. EthnobotLeafl 2009;1:21.Google Scholar ↗

[refR-5] ShendageSM,YadavSR.RevisionofthegenusBarleria(Acanthaceae) in India. Rheedea 2010;20(2):81-130.Google Scholar ↗

[refR-6] ShuklaP, Singh A, Gawri S, Alexande A, Sonwane S. In vitro propagation of BarleriaprionitisLinn and its antibacterial activity. IntJ Pharm Prof Res2011;2:198-200.Google Scholar ↗

[refR-7] KhareCP. Indian Medicinal Plants: An Illustrated Dictionary. 1sted. New York: Springer Sciences; 2007. p.82-3.Google Scholar ↗

[refR-8] ChopraRN,NayarSL,ChopraIC.GlossaryofIndianMedicinalPlants. New Delhi: CSIR; 1956. p.33-4.Google Scholar ↗

[refR-9] AmbastaSP. The Useful Plants of India. New Delhi: CSIR;1986.Google Scholar ↗

[refR-10] BhallaNP, Sahu TR, Mishra GP, Dakwala RN. . J EconTaxon BotGoogle Scholar ↗

[refR-11] ;3:23.Google Scholar ↗

[refR-12] Jain SK,Defillips RA. Medicinal Plants of India. New Delhi: CSIR; 1991.Google Scholar ↗

[refR-13] Patel BK, Chandel BS, Chauhan HC, Patel KB, Parth FM, Patel MV, et al. Evaluation of antibacterial activities of BarleriaprionitisLinn. Afr J Microbiol Res 2015;9Suppl30:1840-8.Google Scholar ↗

[refR-14] Kumar U, Ahmed F, KhanojiaP, Kukreja K, Kumari S, Bhat RA. Exploration of antioxidant and antibacterial activity of BarleriaprionitisLinn. Int J CurrMicrobiolApplSci2013;2(12):585-91.Google Scholar ↗

[refR-15] AiswaryaT, Ravikumar R. A comparitive study on phytochemical analysis, antibacterial activity and antioxidant activity of Barleriaprionitisleavesextractofpetroleumetherandethanolextract.IntJGoogle Scholar ↗

[refR-16] Kalhari KS, Zahida S, Udenigwea CC, Akhtara S, Ata A, Samarasekera R. Glutathione S-transferase, acetylcholinesterase inhibitory and antibacterial activities of chemical constituents of Barleriaprionitis. Z Naturforsch2007;62(b):580-6.Google Scholar ↗

[refR-17] Aneja KR, Joshi R, Sharma C. Potency of BarleriaprionitisL, bark extracts against oral diseases causing strains of bacteria and fungi of clinical origin. N Y Sci J2010;3:5-12.Google Scholar ↗

[refR-18] Khare D, Tiwari KL. Effect of leaf exudates and leaf tissue sap of BarleriaprionitisL,onsporegerminationofsomespicesofanamorphic fungi. Int J Res EngApplSci2016;6(4):1-7.Google Scholar ↗

[refR-19] ChenJL,BlancP,StoddartCA,BoganM,RozhonEJ,ParkinsonN,etal. New iridoids from the medicinal plant Barleriaprionitiswith potent activity against respiratory syncytial virus. J Nat Prod1998;61:1295-7.Google Scholar ↗

[refR-20] KaurR,KaurG,KapoorA.Preliminaryphytochemicalscreeningandin vitro anthelmintic activity of whole plant extracts of BarleriaprionitisLinn,againstearthworms:Pheretimaposthuma.WorldJPharmPharmSci 2015;4Suppl7:1340-7.Google Scholar ↗

[refR-21] Chavana CB, Hogadeb MG, Bhingea SD, Kumbhara M, Tamboli A. In vitro anthelmintic activity of fruit extract of BarleriaprionitisLinn, against Pheretimaposthuma. Int J Pharm PharmSci2010;2(3):49-50.Google Scholar ↗

[refR-22] Gupta RS, Kumar P, Dixit VP, DobhalMP. Antifertility studies of the rootextractoftheBarleriaprionitisLinninmalealbinoratswithspecial reference to testicular cell population dynamics. J Ethnopharmacol 2000;70:111-7.Google Scholar ↗

[refR-23] PradhanDK,MishraMR,MishraA,PandaAK,BeheraR,JhaS,etal. A comprehensive review of plants used as contraceptives. Int J Pharm Sci Res2012;4:148-55.Google Scholar ↗

[refR-24] RavichandranV, Arunachalam G, Subramanian N, Suresh B. Contraception and its significance in traditional system of medicines. Int J Pharm Sci 2009;1Suppl1:1-21.Google Scholar ↗

[refR-25] Kaur R, Sharma A, Kumar R, Kharb R. Rising trends towards herbal contraceptives. J Nat Prod Plant Resour2011;1(4):5-12.Google Scholar ↗

[refR-26] Singh K, Gupta RS. Antifertility activity of β-sisolated from Barleriaprionitis(l), roots in male albino rats. Int J Pharm PharmSci 2016;8(5):88-96.Google Scholar ↗